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Journal articleCho KH, Byrne B, Chae PS, 2013,
Hemifluorinated Maltose-Neopentyl Glycol (HF-MNG) Amphiphiles for Membrane Protein Stabilisation
, CHEMBIOCHEM, Vol: 14, Pages: 452-455, ISSN: 1439-4227- Author Web Link
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- Citations: 31
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Journal articleChappell J, Jensen K, Freemont PS, 2013,
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
, Nucleic Acids Research, Vol: 41, Pages: 3471-3481, ISSN: 0305-1048A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which are inherently time-consuming. To address this, we have validated a completely in vitro approach for the characterization of DNA regulatory elements using Escherichia coli extract cell-free systems. Importantly, we demonstrate that characterization in cell-free systems correlates and is reflective of performance in vivo for the most frequently used DNA regulatory elements. Moreover, we devise a rapid and completely in vitro method to generate DNA templates for cell-free systems, bypassing the need for DNA template generation and amplification from living cells. This in vitro approach is significantly quicker than current characterization methods and is amenable to high-throughput techniques, providing a valuable tool for rapidly prototyping libraries of DNA regulatory elements for synthetic biology.
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Journal articleCurry S, 2013,
Open access: brave new world requires bravery
, Insights, Vol: 26, Pages: 22-27, ISSN: 2048-7754 -
Journal articleFaunce TA, Lubitz W, Rutherford AWB, et al., 2013,
Energy and environment policy case for a global project on artificial photosynthesis
, ENERGY & ENVIRONMENTAL SCIENCE, Vol: 6, Pages: 695-698, ISSN: 1754-5692- Author Web Link
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- Citations: 231
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Journal articleBill R, Byrne B, 2013,
Foreword for EDICT Special Edition, volume 2
, MOLECULAR MEMBRANE BIOLOGY, Vol: 30, Pages: 113-113, ISSN: 0968-7688 -
Journal articleRuggiero A, Marchant J, Squeglia F, et al., 2013,
Molecular determinants of inactivation of the resuscitation promoting factor B from Mycobacterium tuberculosis
, JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, Vol: 31, Pages: 195-205, ISSN: 0739-1102- Author Web Link
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- Citations: 30
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Journal articleXu Y, Matthews S, 2013,
MAP-XSII: an improved program for the automatic assignment of methyl resonances in large proteins
, JOURNAL OF BIOMOLECULAR NMR, Vol: 55, Pages: 179-187, ISSN: 0925-2738- Author Web Link
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- Citations: 31
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Journal articleWang GD, Heng JYY, Williams DR, 2013,
Dilatometry of powder compacts - Characterizing amorphous-crystalline transformations
, POWDER TECHNOLOGY, Vol: 236, Pages: 12-16, ISSN: 0032-5910- Author Web Link
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- Citations: 4
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Journal articleBill R, Byrne B, 2013,
Introduction to EDICT volume 1
, MOLECULAR MEMBRANE BIOLOGY, Vol: 30, Pages: 2-2, ISSN: 0968-7688 -
Journal articleKhoo JY, Shah UV, Schaepertoens M, et al., 2013,
Process-induced phase transformation of carbamazepine dihydrate to its polymorphic anhydrates
, POWDER TECHNOLOGY, Vol: 236, Pages: 114-121, ISSN: 0032-5910- Author Web Link
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- Citations: 21
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Journal articleDelmas T, Shah UV, Roberts MM, et al., 2013,
Crystallisation of the orthorhombic form of acetaminophen: Combined effect of surface topography and chemistry
, POWDER TECHNOLOGY, Vol: 236, Pages: 24-29, ISSN: 0032-5910- Author Web Link
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- Citations: 16
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Journal articleSolomon A, Smyth E, Mitha N, et al., 2013,
Induction of platelet aggregation after a direct physical interaction with diesel exhaust particles
, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 11, Pages: 325-334, ISSN: 1538-7933- Author Web Link
- Open Access Link
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- Citations: 21
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Journal articlePorta C, Xu X, Loureiro S, et al., 2013,
Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity
, JOURNAL OF VIROLOGICAL METHODS, Vol: 187, Pages: 406-412, ISSN: 0166-0934- Author Web Link
- Open Access Link
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- Citations: 42
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Conference paperZhao Z, Rutherford AW, Moser CC, et al., 2013,
Photosynthetic Reaction Center Performance under Physiologically Relevant Energetic Changes
, 57th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 489A-489A, ISSN: 0006-3495 -
Journal articleSimon AC, Simpson PJ, Goldstone RM, et al., 2013,
Structure of the Sgt2/Get5 complex provides insights into GET-mediated targeting of tail-anchored membrane proteins
, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 1327-1332, ISSN: 0027-8424- Author Web Link
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- Citations: 25
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Journal articleSaito K, Rutherford AW, Ishikita H, 2013,
Mechanism of proton-coupled quinone reduction in Photosystem II
, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 954-959, ISSN: 0027-8424- Author Web Link
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- Citations: 104
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Journal articleMesquita FS, Holden DW, Rolhion N, 2013,
Lack of Effect of the <i>Salmonella</i> Deubiquitinase SseL on the NF-κB Pathway
, PLOS ONE, Vol: 8, ISSN: 1932-6203- Author Web Link
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- Citations: 17
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Journal articleThapper A, Styring S, Saracco G, et al., 2013,
Artificial photosynthesis for solar fuels - An evolving research field within AMPEA, a joint programme of the European energy research alliance
, Green, Vol: 3, Pages: 43-57, ISSN: 1869-876XOn the path to an energy transition away from fossil fuels to sustainable sources, the European Union is for the moment keeping pace with the objectives of the Strategic Energy Technology-Plan. For this trend to continue after 2020, scientific breakthroughs must be achieved. One main objective is to produce solar fuels from solar energy and water in direct processes to accomplish the efficient storage of solar energy in a chemical form. This is a grand scientific challenge. One important approach to achieve this goal is Artificial Photosynthesis. The European Energy Research Alliance has launched the Joint Programme "Advanced Materials & Processes for Energy Applications" (AMPEA) to foster the role of basic science in Future Emerging Technologies. European researchers in artificial photosynthesis recently met at an AMPEA organized workshop to define common research strategies and milestones for the future. Through this work artificial photosynthesis became the first energy research sub-field to be organised into what is designated "an Application" within AMPEA. The ambition is to drive and accelerate solar fuels research into a powerful European field - in a shorter time and with a broader scope than possible for individual or national initiatives. Within AMPEA the Application Artificial Photosynthesis is inclusive and intended to bring together all European scientists in relevant fields. The goal is to set up a thorough and systematic programme of directed research, which by 2020 will have advanced to a point where commercially viable artificial photosynthetic devices will be under development in partnership with industry.
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Journal articleAbrusci P, Vergara-Irigaray M, Johnson S, et al., 2013,
Architecture of the major component of the type III secretion system export apparatus
, NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 20, Pages: 99-U126, ISSN: 1545-9993- Author Web Link
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- Citations: 164
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Journal articleMetifiot M, Maddali K, Johnson BC, et al., 2013,
Activities, Crystal Structures, and Molecular Dynamics of Dihydro-1<i>H</i>-isoindole Derivatives, Inhibitors of HIV-1 lntegrase
, ACS CHEMICAL BIOLOGY, Vol: 8, Pages: 209-217, ISSN: 1554-8929- Author Web Link
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- Citations: 40
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Journal articleBebeacua C, Lai L, Vegge CS, et al., 2013,
Visualizing a Complete <i>Siphoviridae</i> Member by Single-Particle Electron Microscopy: the Structure of Lactococcal Phage TP901-1
, JOURNAL OF VIROLOGY, Vol: 87, Pages: 1061-1068, ISSN: 0022-538X- Author Web Link
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- Citations: 49
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Journal articleKaucikas M, Warren M, Michailovas A, et al., 2013,
Beam patterns in OPO employing walk-off compensating BBO crystals
, Laser Physics, Pages: 1-6 -
Journal articleWarren M, Kaucikas M, Fitzpatrick A, et al., 2013,
Ground state proton transfer in the photoswitching reactions of the fluorescent protein Dronpa
, Nature Communications -
Journal articleKaucikas M, Barber J, van Thor JJ, 2013,
Polarization sensitive ultrafast mid-IR pump probe micro-spectrometer with diffraction limited spatial resolution
, Optics Express, Vol: 21, Pages: 8357-8370 -
Book chapterKim TW, Lee JH, Choi J, et al., 2013,
Unravelling the protein structural dynamics of photoactive yellow protein in solution using pump-probe X-ray solution scattering
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Journal articleBoehm CR, Freemont PS, Ces O, 2013,
Design of a prototype flow microreactor for synthetic biology <i>in vitro</i>
, LAB ON A CHIP, Vol: 13, Pages: 3426-3432, ISSN: 1473-0197- Author Web Link
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- Citations: 25
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Book chapterChoudhury HG, Beis K, 2013,
Tellurite-Detoxifying Protein TehB from Escherichia coli
, Encyclopedia of Metalloproteins, Editors: Kretsinger, Uversky, Permyakov, Publisher: Springer, ISBN: 978-1-4614-1532-9 -
Book chapterChoudhury HG, Beis K, 2013,
Tellurite-Resistance Protein TehA from Escherichia coli
, Encyclopedia of Metalloproteins, Editors: Kretsinger, Uversky, Permyakov, Publisher: Springer, ISBN: 978-1-4614-1532-9 -
BookMatthews S, Heise H, 2013,
Modern NMR Methodology Preface
, Publisher: SPRINGER-VERLAG BERLIN -
Journal articleChappell J, Freemont P, 2013,
In Vivo and In Vitro Characterization of σ<SUP>70</SUP> Constitutive Promoters by Real-Time PCR and Fluorescent Measurements
, SYNTHETI C BIOLOGY, Vol: 1073, Pages: 61-74, ISSN: 1064-3745- Author Web Link
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- Citations: 3
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