Citation

BibTex format

@article{Bayer:2016:10.1016/j.chom.2016.10.007,
author = {Bayer, Santos E and Durkin, CH and Rigano, L and Kupz, A and Alix, E and Cerny, O and Jennings, E and Liu, M and Ryan, A and Lapaque, N and Kaufmann, S and Holden, D and Robb, C},
doi = {10.1016/j.chom.2016.10.007},
journal = {Cell Host & Microbe},
pages = {584--595},
title = {The Salmonella effector SteD mediates MARCH8-1 dependent ubiquitination of MHC II molecules and inhibits T cell activation},
url = {http://dx.doi.org/10.1016/j.chom.2016.10.007},
volume = {20},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The SPI-2 type III secretion system (T3SS) of intracellular Salmonella enterica translocates effector proteins into mammalian cells. Infection of antigen-presenting cells results in SPI-2 T3SS-dependent ubiquitination and reduction of surface-localized mature MHC class II (mMHCII). We identify the effector SteD as required and sufficient for this process. In Mel Juso cells, SteD localized to the Golgi network and vesicles containing the E3 ubiquitin ligase MARCH8 and mMHCII. SteD caused MARCH8-dependent ubiquitination and depletion of surface mMHCII. One of two transmembrane domains and the C-terminal cytoplasmic region of SteD mediated binding to MARCH8 and mMHCII, respectively. Infection of dendritic cells resulted in SteD-dependent depletion of surface MHCII, the co-stimulatory molecule B7.2, and suppression of T cell activation. SteD also accounted for suppression of T cell activation during Salmonella infection of mice. We propose that SteD is an adaptor, forcing inappropriate ubiquitination of mMHCII by MARCH8 and thereby suppressing T cell activation.
AU  - Bayer,Santos E
AU  - Durkin,CH
AU  - Rigano,L
AU  - Kupz,A
AU  - Alix,E
AU  - Cerny,O
AU  - Jennings,E
AU  - Liu,M
AU  - Ryan,A
AU  - Lapaque,N
AU  - Kaufmann,S
AU  - Holden,D
AU  - Robb,C
DO  - 10.1016/j.chom.2016.10.007
EP  - 595
PY  - 2016///
SN  - 1934-6069
SP  - 584
TI  - The Salmonella effector SteD mediates MARCH8-1 dependent ubiquitination of MHC II molecules and inhibits T cell activation
T2  - Cell Host & Microbe
UR  - http://dx.doi.org/10.1016/j.chom.2016.10.007
UR  - https://www.sciencedirect.com/science/article/pii/S1931312816304334?via%3Dihub
UR  - http://hdl.handle.net/10044/1/41746
VL  - 20
ER  -
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