BibTex format
@article{Pruski:2021:10.1038/s41467-021-26215-w,
author = {Pruski, P and Dos, Santos Correia G and Lewis, H and Capuccini, K and Inglese, P and Chan, D and Brown, R and Kindinger, L and Lee, Y and Smith, A and Marchesi, J and McDonald, J and Cameron, S and Alexander-Hardiman, K and David, A and Stock, S and Norman, J and Terzidou, V and Teoh, TG and Sykes, L and Bennett, P and Takats, Z and MacIntyre, D},
doi = {10.1038/s41467-021-26215-w},
journal = {Nature Communications},
title = {Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth},
url = {http://dx.doi.org/10.1038/s41467-021-26215-w},
volume = {12},
year = {2021}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics.
AU - Pruski,P
AU - Dos,Santos Correia G
AU - Lewis,H
AU - Capuccini,K
AU - Inglese,P
AU - Chan,D
AU - Brown,R
AU - Kindinger,L
AU - Lee,Y
AU - Smith,A
AU - Marchesi,J
AU - McDonald,J
AU - Cameron,S
AU - Alexander-Hardiman,K
AU - David,A
AU - Stock,S
AU - Norman,J
AU - Terzidou,V
AU - Teoh,TG
AU - Sykes,L
AU - Bennett,P
AU - Takats,Z
AU - MacIntyre,D
DO - 10.1038/s41467-021-26215-w
PY - 2021///
SN - 2041-1723
TI - Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-021-26215-w
UR - http://hdl.handle.net/10044/1/92499
VL - 12
ER -
