BibTex format
@article{Parsons:2015:10.1016/j.cell.2015.06.060,
author = {Parsons, MJ and Brancaccio, M and Sethi, S and Maywood, ES and Satija, R and Edwards, JK and Jagannath, A and Couch, Y and Finelli, MJ and Smyllie, NJ and Esapa, C and Butler, R and Barnard, AR and Chesham, JE and Saito, S and Joynson, G and Wells, S and Foster, RG and Oliver, PL and Simon, MM and Mallon, A-M and Hastings, MH and Nolan, PM},
doi = {10.1016/j.cell.2015.06.060},
journal = {Cell},
pages = {607--621},
title = {The regulatory factor ZFHX3 modifies circadian function in SCN via an AT motif-driven axis},
url = {http://dx.doi.org/10.1016/j.cell.2015.06.060},
volume = {162},
year = {2015}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - We identified a dominant missense mutation in the SCN transcription factor Zfhx3, termed short circuit (Zfhx3Sci), which accelerates circadian locomotor rhythms in mice. ZFHX3 regulates transcription via direct interaction with predicted AT motifs in target genes. The mutant protein has a decreased ability to activate consensus AT motifs in vitro. Using RNA sequencing, we found minimal effects on core clock genes in Zfhx3Sci/+ SCN, whereas the expression of neuropeptides critical for SCN intercellular signaling was significantly disturbed. Moreover, mutant ZFHX3 had a decreased ability to activate AT motifs in the promoters of these neuropeptide genes. Lentiviral transduction of SCN slices showed that the ZFHX3-mediated activation of AT motifs is circadian, with decreased amplitude and robustness of these oscillations in Zfhx3Sci/+ SCN slices. In conclusion, by cloning Zfhx3Sci, we have uncovered a circadian transcriptional axis that determines the period and robustness of behavioral and SCN molecular rhythms.
AU - Parsons,MJ
AU - Brancaccio,M
AU - Sethi,S
AU - Maywood,ES
AU - Satija,R
AU - Edwards,JK
AU - Jagannath,A
AU - Couch,Y
AU - Finelli,MJ
AU - Smyllie,NJ
AU - Esapa,C
AU - Butler,R
AU - Barnard,AR
AU - Chesham,JE
AU - Saito,S
AU - Joynson,G
AU - Wells,S
AU - Foster,RG
AU - Oliver,PL
AU - Simon,MM
AU - Mallon,A-M
AU - Hastings,MH
AU - Nolan,PM
DO - 10.1016/j.cell.2015.06.060
EP - 621
PY - 2015///
SN - 0092-8674
SP - 607
TI - The regulatory factor ZFHX3 modifies circadian function in SCN via an AT motif-driven axis
T2 - Cell
UR - http://dx.doi.org/10.1016/j.cell.2015.06.060
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000358801800019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
VL - 162
ER -