In this section
@inproceedings{Forsyth:1996:10.1159/000111429,
author = {Forsyth, R and Fray, A and Boutelle, M and Fillenz, M and Middleditchc, C and Burchell, A},
doi = {10.1159/000111429},
pages = {360--370},
title = {A role for astrocytes in glucose delivery to neurons?},
url = {http://dx.doi.org/10.1159/000111429},
year = {1996}
}
TY - CPAPER
AB - The present paper examines the possible role of astrocytes in the delivery of glycogen-derived glucose for neuronal metabolism. Such a process would require astrocytic expression of glucose-6-phosphatase. The degree and significance of brain expression of glucose-6-phosphatase (EC 3.1.3.9) has been a subject of controversy. Published immunohistochemical data are consistent with expression of glucose-6-phosphatase by astrocytes, both in vivo and in vitro. In this paper additional confirmation of the expression of glucose-6-phosphatase mRNA in rat brain is presented. Although cultured astrocytes demonstrate glucose-6-phosphatase activity in vitro under assay conditions, there is very limited in vitro evidence that this activity confers a glucose-export capacity on astrocytes. Under most conditions in vitro, lactate export predominates, however this may relate to aspects of the in vitro phenotype. Data relating to astrocytic glucose and lactate export are considered in the context of hypotheses of trafficking by astrocytes of substrates for neuronal metabolism, hypotheses that imply and require compartmentation of these substances, in contrast with current formulations of glucose transport into and within brain that imply no glucose compartmentation. Microdialysis studies of the properties of the brain extracellular fluid (ECF) glucose pool in the freely moving rat were performed seeking evidence of glucose compartmentation. Results of these studies do imply compartmentalisation of brain glucose, and are consistent with a model envisaging the majority of glucose reaching the neuron via the astrocytic intracellular space and the ECF. In addition, such studies provide evidence that rises in ECF glucose concentration are not the direct result of local recruitment of cerebral blood flow, but suggest the influence of intermediate, astrocyte-based mechanisms. Astrocytic glucose-6-phosphatase may permit astrocytes to modulate the transastrocytic flux of glucose to adjacent ne
AU - Forsyth,R
AU - Fray,A
AU - Boutelle,M
AU - Fillenz,M
AU - Middleditchc,C
AU - Burchell,A
DO - 10.1159/000111429
EP - 370
PY - 1996///
SN - 0378-5866
SP - 360
TI - A role for astrocytes in glucose delivery to neurons?
UR - http://dx.doi.org/10.1159/000111429
ER -
Professor Martyn Boutelle
Department of Bioengineering
Room 3.14, Royal School of Mines Building
Imperial College London
London, SW7 2AZ
Tel: +44 (0)20 7594 5138
Email: m.boutelle@imperial.ac.uk
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