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@article{Fricker:2018:10.1007/978-1-4939-7389-7_5,
author = {Fricker, M and Heaton, L and Jones, N and Obara, B and Müller, SJ and Meyer, AJ},
doi = {10.1007/978-1-4939-7389-7_5},
journal = {Methods Mol Biol},
pages = {43--66},
title = {Quantitation of ER Structure and Function.},
url = {http://dx.doi.org/10.1007/978-1-4939-7389-7_5},
volume = {1691},
year = {2018}
}
TY - JOUR
AB - The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a 2-D planar network. The structure of the network, and the morphology of the tubules and cisternae can be automatically extracted following intensity-independent edge-enhancement and various segmentation techniques to give an initial pixel-based skeleton, which is then converted to a graph representation. Collectively, this approach yields a wealth of quantitative metrics for ER structure and can be used to describe the effects of pharmacological treatments or genetic manipulation. The software is publicly available.
AU - Fricker,M
AU - Heaton,L
AU - Jones,N
AU - Obara,B
AU - Müller,SJ
AU - Meyer,AJ
DO - 10.1007/978-1-4939-7389-7_5
EP - 66
PY - 2018///
SP - 43
TI - Quantitation of ER Structure and Function.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1007/978-1-4939-7389-7_5
UR - https://www.ncbi.nlm.nih.gov/pubmed/29043669
VL - 1691
ER -