BibTex format
@article{Toumazou:2013:10.1038/nmeth.2520,
author = {Toumazou, C and Shepherd, LM and Reed, S and Chen, GI and Patel, A and Garner, DM and Wang, CA and Ou, CP and Amin-Desai, K and Athanasiou, P and Bai, H and Brizido, IMQ and Caldwell, B and Coomber-Alford, D and Georgiou, P and Jordan, KS and Joyce, JC and La, Mura M and Morley, D and Sathyavruthan, S and Temelso, S and Temelso, RE and Zhang, L},
doi = {10.1038/nmeth.2520},
journal = {Nature Methods},
title = {Simultaneous DNA amplification and detection using a pH-sensing semiconductor system},
url = {http://dx.doi.org/10.1038/nmeth.2520},
volume = {10},
year = {2013}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - We developed an integrated chip for real-time amplification and detection of nucleic acid using pH-sensing complementary metal-oxide semiconductor (CMOS) technology. Here we show an amplification-coupled detection method for directly measuring released hydrogen ions during nucleotide incorporation rather than relying on indirect measurements such as fluorescent dyes. This is a label-free, non-optical, real-time method for detecting and quantifying target sequences by monitoring pH signatures of native amplification chemistries. The chip has ion-sensitive field effect transistor (ISFET) sensors, temperature sensors, resistive heating, signal processing and control circuitry all integrated to create a full system-on-chip platform. We evaluated the platform using two amplification strategies: PCR and isothermal amplification. Using this platform, we genotyped and discriminated unique single-nucleotide polymorphism (SNP) variants of the cytochrome P450 family from crude human saliva. We anticipate this semiconductor technology will enable the creation of devices for cost-effective, portable and scalable real-time nucleic acid analysis.
AU - Toumazou,C
AU - Shepherd,LM
AU - Reed,S
AU - Chen,GI
AU - Patel,A
AU - Garner,DM
AU - Wang,CA
AU - Ou,CP
AU - Amin-Desai,K
AU - Athanasiou,P
AU - Bai,H
AU - Brizido,IMQ
AU - Caldwell,B
AU - Coomber-Alford,D
AU - Georgiou,P
AU - Jordan,KS
AU - Joyce,JC
AU - La,Mura M
AU - Morley,D
AU - Sathyavruthan,S
AU - Temelso,S
AU - Temelso,RE
AU - Zhang,L
DO - 10.1038/nmeth.2520
PY - 2013///
SN - 1548-7105
TI - Simultaneous DNA amplification and detection using a pH-sensing semiconductor system
T2 - Nature Methods
UR - http://dx.doi.org/10.1038/nmeth.2520
VL - 10
ER -