BibTex format
@article{Pallett:2022:10.2807/1560-7917.ES.2022.27.4.2002076,
author = {Pallett, SJ and Jones, R and Abdulaal, A and Pallett, MA and Rayment, M and Patel, A and Denny, SJ and Mughal, N and Khan, M and de, Oliveira CR and Pantelidis, P and Randell, P and Toumazou, C and O'Shea, MK and Tedder, R and McClure, MO and Davies, GW and Moore, LSP},
doi = {10.2807/1560-7917.ES.2022.27.4.2002076},
journal = {Eurosurveillance},
title = {Variability in detection of SARS-CoV-2-specific antibody responses following mild infection: a prospective multicentre cross-sectional study, London, United Kingdom, 17 April to 17 July 2020},
url = {http://dx.doi.org/10.2807/1560-7917.ES.2022.27.4.2002076},
volume = {27},
year = {2022}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - IntroductionImmunoassays targeting different SARS-CoV-2-specific antibodies are employed for seroprevalence studies. The degree of variability between immunoassays targeting anti-nucleocapsid (anti-NP; the majority) vs the potentially neutralising anti-spike antibodies (including anti-receptor-binding domain; anti-RBD), particularly in mild or asymptomatic disease, remains unclear.AimsWe aimed to explore variability in anti-NP and anti-RBD antibody detectability following mild symptomatic or asymptomatic SARS-CoV-2 infection and analyse antibody response for correlation with symptomatology.MethodsA multicentre prospective cross-sectional study was undertaken (April–July 2020). Paired serum samples were tested for anti-NP and anti-RBD IgG antibodies and reactivity expressed as binding ratios (BR). Multivariate linear regression was performed analysing age, sex, time since onset, symptomatology, anti-NP and anti-RBD antibody BR.ResultsWe included 906 adults. Antibody results (793/906; 87.5%; 95% confidence interval: 85.2–89.6) and BR strongly correlated (ρ = 0.75). PCR-confirmed cases were more frequently identified by anti-RBD (129/130) than anti-NP (123/130). Anti-RBD testing identified 83 of 325 (25.5%) cases otherwise reported as negative for anti-NP. Anti-NP presence (+1.75/unit increase; p < 0.001), fever (≥ 38°C; +1.81; p < 0.001) or anosmia (+1.91; p < 0.001) were significantly associated with increased anti-RBD BR. Age (p = 0.85), sex (p = 0.28) and cough (p = 0.35) were not. When time since symptom onset was considered, we did not observe a significant change in anti-RBD BR (p = 0.95) but did note decreasing anti-NP BR (p < 0.001).ConclusionSARS-CoV-2 anti-RBD IgG showed significant correlation with anti-NP IgG for absolute seroconversion and BR. Higher BR were seen in symptomatic indiv
AU - Pallett,SJ
AU - Jones,R
AU - Abdulaal,A
AU - Pallett,MA
AU - Rayment,M
AU - Patel,A
AU - Denny,SJ
AU - Mughal,N
AU - Khan,M
AU - de,Oliveira CR
AU - Pantelidis,P
AU - Randell,P
AU - Toumazou,C
AU - O'Shea,MK
AU - Tedder,R
AU - McClure,MO
AU - Davies,GW
AU - Moore,LSP
DO - 10.2807/1560-7917.ES.2022.27.4.2002076
PY - 2022///
SN - 1025-496X
TI - Variability in detection of SARS-CoV-2-specific antibody responses following mild infection: a prospective multicentre cross-sectional study, London, United Kingdom, 17 April to 17 July 2020
T2 - Eurosurveillance
UR - http://dx.doi.org/10.2807/1560-7917.ES.2022.27.4.2002076
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000748460500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2022.27.4.2002076#abstract_content
UR - http://hdl.handle.net/10044/1/95043
VL - 27
ER -