Notable Recent Publications

These are some recent publications which give a flavour of the research from the Barclay lab. For a complete list of publications, please see below.

Species difference in ANP32A underlies influenza A virus polymerase host restriction. Nature (2016).
Jason S. Long, Efstathios S. Giotis, Olivier Moncorgé, Rebecca Frise, Bhakti Mistry, Joe James, Mireille Morisson, Munir Iqbal, Alain Vignal, Michael A. Skinner & Wendy S. Barclay

This paper identified a key factor that explained why the polymerases from avian influenza viruses are restricted in humans.  For more, please see the associated New and Views.

See our latest ANP32 papers here: eLIFE, Journal of Virology, Journal of Virology.

The mechanism of resistance to favipiravir in influenza. PNAS (2018).
Daniel H. Goldhill, Aartjan J. W. te Velthuis, Robert A. Fletcher, Pinky Langat, Maria Zambon, Angie Lackenby & Wendy S. Barclay

This paper showed how influenza could evolve resistance to favipiravir, an antiviral that may be used to treat influenza. The residue that mutated to give resistance was highly conserved suggesting that the mechanism of resistance may be applicable to other RNA viruses.

Internal genes of a highly pathogenic H5N1 influenza virus determine high viral replication in myeloid cells and severe outcome of infection in mice. Plos Path. (2018).
Hui Li*, Konrad C. Bradley*, Jason S. Long, Rebecca Frise, Jonathan W. Ashcroft, Lorian C. Hartgroves, Holly Shelton, Spyridon Makris, Cecilia Johansson, Bin Cao & Wendy S. Barclay

Why do avian influenza viruses like H5N1 cause such severe disease in humans? This paper demonstrated that H5N1 viruses replicate better than human viruses in myeloid cells from mice leading to a cytokine storm and more severe disease.

Citation

BibTex format

@article{Cole:2017:10.1172/jci.insight.91868,
author = {Cole, SL and Dunning, J and Kok, WL and Benam, KH and Benlahrech, A and Repapi, E and Martinez, FO and Drumright, L and Powell, TJ and Bennett, M and Elderfield, R and Thomas, C and MOSAIC, investigators and Dong, T and McCauley, J and Liew, FY and Taylor, S and Zambon, M and Barclay, W and Cerundolo, V and Openshaw, PJ and McMichael, AJ and Ho, LP},
doi = {10.1172/jci.insight.91868},
journal = {JCI Insight},
title = {M1-like monocytes are a major immunological determinant of severity in previously healthy adults with life-threatening influenza.},
url = {http://dx.doi.org/10.1172/jci.insight.91868},
volume = {2},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In each influenza season, a distinct group of young, otherwise healthy individuals with no risk factors succumbs to life-threatening infection. To better understand the cause for this, we analyzed a broad range of immune responses in blood from a unique cohort of patients, comprising previously healthy individuals hospitalized with and without respiratory failure during one influenza season, and infected with one specific influenza A strain. This analysis was compared with similarly hospitalized influenza patients with known risk factors (total of n = 60 patients recruited). We found a sustained increase in a specific subset of proinflammatory monocytes, with high TNF-α expression and an M1-like phenotype (independent of viral titers), in these previously healthy patients with severe disease. The relationship between M1-like monocytes and immunopathology was strengthened using murine models of influenza, in which severe infection generated using different models (including the high-pathogenicity H5N1 strain) was also accompanied by high levels of circulating M1-like monocytes. Additionally, a raised M1/M2 macrophage ratio in the lungs was observed. These studies identify a specific subtype of monocytes as a modifiable immunological determinant of disease severity in this subgroup of severely ill, previously healthy patients, offering potential novel therapeutic avenues.
AU  - Cole,SL
AU  - Dunning,J
AU  - Kok,WL
AU  - Benam,KH
AU  - Benlahrech,A
AU  - Repapi,E
AU  - Martinez,FO
AU  - Drumright,L
AU  - Powell,TJ
AU  - Bennett,M
AU  - Elderfield,R
AU  - Thomas,C
AU  - MOSAIC,investigators
AU  - Dong,T
AU  - McCauley,J
AU  - Liew,FY
AU  - Taylor,S
AU  - Zambon,M
AU  - Barclay,W
AU  - Cerundolo,V
AU  - Openshaw,PJ
AU  - McMichael,AJ
AU  - Ho,LP
DO  - 10.1172/jci.insight.91868
PY  - 2017///
SN  - 2379-3708
TI  - M1-like monocytes are a major immunological determinant of severity in previously healthy adults with life-threatening influenza.
T2  - JCI Insight
UR  - http://dx.doi.org/10.1172/jci.insight.91868
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28405622
UR  - http://hdl.handle.net/10044/1/48142
VL  - 2
ER  -
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Contact us

For any enquiries related to this group, please contact:

Professor Wendy Barclay
Chair in Influenza Virology 
+44 (020) 7594 5035
w.barclay@imperial.ac.uk