In this section
@article{Malpartida-Cardenas:2022:10.1039/d2sd00038e,
author = {Malpartida-Cardenas, K and Miglietta, L and Peng, T and Moniri, A and Holmes, A and Georgiou, P and Rodriguez-Manzano, J},
doi = {10.1039/d2sd00038e},
journal = {Sensors and Diagnostics},
pages = {465--468},
title = {Single-channel digital LAMP multiplexing using Amplification Curve Analysis},
url = {http://dx.doi.org/10.1039/d2sd00038e},
volume = {1},
year = {2022}
}
TY - JOUR
AB - Loop-mediated isothermal amplification assays are currently limited to one target per reaction in the absence of melting curve analysis, molecular probes or restriction enzyme digestion. Here, we demonstrate multiplexing of five targets in a single fluorescent channel using digital LAMP and the machine learning-based method amplification curve analysis, resulting in a classification accuracy of 91.33% on 54186 positive amplification events.
AU - Malpartida-Cardenas,K
AU - Miglietta,L
AU - Peng,T
AU - Moniri,A
AU - Holmes,A
AU - Georgiou,P
AU - Rodriguez-Manzano,J
DO - 10.1039/d2sd00038e
EP - 468
PY - 2022///
SN - 2635-0998
SP - 465
TI - Single-channel digital LAMP multiplexing using Amplification Curve Analysis
T2 - Sensors and Diagnostics
UR - http://dx.doi.org/10.1039/d2sd00038e
UR - https://pubs.rsc.org/en/content/articlelanding/2022/SD/D2SD00038E
UR - http://hdl.handle.net/10044/1/96559
VL - 1
ER -
