BibTex format
@article{Barretto:2016:10.1016/j.bej.2016.10.007,
author = {Barretto, S and Michoux, F and Hellgardt, K and Nixon, PJ},
doi = {10.1016/j.bej.2016.10.007},
journal = {Biochemical Engineering Journal},
pages = {73--81},
title = {Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during in vitro shoot regeneration of Nicotiana tabacum callus: Implications for bioprocess routes to plant-made biopharmaceuticals},
url = {http://dx.doi.org/10.1016/j.bej.2016.10.007},
volume = {11},
year = {2016}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Transplastomic plants are capable of high-yield production of recombinant biopharmaceutical proteins. Planttissue culture combines advantages of agricultural cultivation with the bioprocess consistency associated withsuspension culture. Overexpression of recombinant proteins through regeneration of transplastomic Nicotianatabacum shoots from callus tissue in RITA® temporary immersion bioreactors has been previously demonstrated.In this study we investigated the hydrodynamics of periodic pneumatic suspension of liquid medium duringtemporary immersion culture (4 minutes aeration every 8 hours), and the impact on biological responses andtransplastomic expression of fragment C of tetanus toxin (TetC). Biomass was grown under a range of aerationrates for 3, 20 and 40-day durations. Growth, mitochondrial activity (a viability indicator) and TetC protein yieldswere correlated against the hydrodynamic parameters, shear rate and energy dissipation rate (per kg of medium).A critical aeration rate of 440 ml min-1 was identified, corresponding to a shear rate of 96.7 s-1, pneumatic powerinput of 8.8 mW kg-1and initial 20-day pneumatic energy dissipation of 127 J kg-1, at which significant reductionsin biomass accumulation and mitochondrial activity were observed. There was an exponential decline in TetCyields with increasing aeration rates at 40 days, across the entire range of conditions tested. These observationshave important implications for the optimisation and scale-up of transplastomic plant tissue culture bioprocessesfor biopharmaceutical production.
AU - Barretto,S
AU - Michoux,F
AU - Hellgardt,K
AU - Nixon,PJ
DO - 10.1016/j.bej.2016.10.007
EP - 81
PY - 2016///
SN - 1369-703X
SP - 73
TI - Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during in vitro shoot regeneration of Nicotiana tabacum callus: Implications for bioprocess routes to plant-made biopharmaceuticals
T2 - Biochemical Engineering Journal
UR - http://dx.doi.org/10.1016/j.bej.2016.10.007
UR - http://hdl.handle.net/10044/1/41427
VL - 11
ER -